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Cloning and Expression
Enzymes
SacI
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SacI
SacI
SacI from Promega
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Citations:
(4)
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SacI
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Description
G AGCT/C C\TCGA G
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
SacI
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
R6061
Quantity
1,000u
Concentration
10u/µl
Molecular Weight
2,765±3 a.m.u.
Sequence
G AGCT▼C
C▲TCGA G
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Promega
2800 Woods Hollow Rd.
Madison
,
Wisconsin
United States
Phone
: 800-356-9526
Fax: 800-356-1970
Company Profile
Website:
http://www.promega.com
Citations (4)
(1)
Pesticide residue exposure provides different responses of the microbiomes of distinct cultures of the stored product pest mite Acarus siro
BMC Microbiology
October 19, 2022
Jan Hubert, Blanka Navratilova, Bruno Sopko, Marta Nesvorna, Thomas W. Phillips
Promega
) according to the manufacturer’s protocol. Plasmids were linearized by
SacI
restriction (Cat. No.
R6061
,
Promega
) and cleaned with a
Wizard SV gel and PCR Clean-Up system
(Cat No.
A9285
). The concentration
(2)
Suberanilohydroxamic acid prevents TGF-β1-induced COX-2 repression in human lung fibroblasts post-transcriptionally by TIA-1 downregulation
Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms
May 1, 2018
Alice Pasini, Oliver J. Brand, Gisli Jenkins, Alan J. Knox, Linhua Pang
reverse 5′-CTCTCTCGAGTTTCCAACACAGTGTCGCAG-3′. 1 μg of purified
PCR
products was then digested using
SacI
(
R6061
,
Promega
) and
XhoI
(
R6161
,
Promega
) restriction enzymes following the manufacturer's instructions. Digestion
(3)
Intracellular uptake of macromolecules by brain lymphatic endothelial cells during zebrafish embryonic development
eLife
May 12, 2017
van Lessen M, Shibata-Germanos S, van Impel A, Hawkins TA, Rihel J, Schulte-Merker S.
mutants was performed by
PCR amplification
(Table 1) followed by
restriction digest
with
SacI
(
Promega
, #
R6061
). Analysis was performed by standard
gel electrophoresis
. Live imaging Live imaging was carried out
(4)
A novel adjuvant-free H fusion system for the production of recombinant immunogens in Escherichia coli: Its application to a 12 kDa antigen from Cryptosporidium parvum
Bioengineered
November 1, 2013
Sofia J Costa, Pedro Silva, André Almeida, Antónia Conceição, Lucília Domingues, António Castro
plasmid containing the cp12 gene was digested with
SacI
and
KpnI
restriction enzymes (
Promega
, Catalog #
R6061
and
R6341
) and the cp12 gene was then cloned into the same restriction sites of pQE-30 (
Qiagen
, Catalog
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SacI from Promega
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